The percentage reduction of scaffold material was calculated by taking the fluorescent area at week 1 as 100% and subtracting the fluorescent area percentage at week 6, as described in the Materials and Methods. ( B) Quantitative data of the degradation rate of the two differently designed scaffolds. Ctr: control group CHPA: Scaffold material + Vehicle CHPA + BMP-2: Scaffold material + BMP-2 CHPA + OP3-4: Scaffold material + OP3-4 CHPA + BMP-2 + OP3-4: Scaffold material+BMP-2 + OP3-4. Excitation/absorption wavelengths: 560/620 nm color scale: minimum 2.55 × 10 9, maximum 5.17 × 10 9. Yellow areas in the figure represent the location and fluorescence intensity of rhodamine-bound CHPA. Fluorescent sites and intensities of rhodamine-bound scaffold material at 1 and 6 weeks, after scaffold placement. Perforated CHPA scaffold showed a slower degradation rate than nonperforated CHPA: ( A) In vivo images of IVIS. These data suggest that perforated CHPA nanogel could lead to local bone formation induced by OP3-4 and BMP-2 and clarified the appropriate degradation rate for inducing local bone formation when CHPA nanogels are designed to be perforated.īMP-2 CHPA OP3-4 RANKL-binding peptide and nonperforated scaffold bone regeneration cholesterol-bearing pullulan perforated scaffold. The degradation rate of scaffold material in the perforated OP3-4/BMP-2 combination group was lower than that in the nonperforated group. A higher cortical bone mineral content and bone formation rate were observed in the perforated scaffold in comparison to the nonperforated scaffold, especially in the OP3-4/BMP-2 combination group. The mice were euthanized at 6 weeks postoperatively. Thirty-six, five-week-old male BALB/c mice were used for the calvarial defect model. We also clarified the difference between perforated and nonperforated CHPA impregnated with the two signaling molecules. In the present study, we investigated the osteoconductive capacity of a newly synthesized CHP nanogel, CHPA using OP3-4 and BMP-2. Cholesterol-bearing pullulan (CHP) nanogel scaffold has been shown to prevent aggregation of peptides and to allow their sustained release and activity however, the appropriate design of CHP nanogels to conduct local bone formation needs to be developed.
#Protein scaffold trachea Activator#
The receptor activator of NF-κB ligand (RANKL)-binding peptide, OP3-4, is known to stimulate bone morphogenetic protein (BMP)-2-induced bone formation, but peptides tend to aggregate and lose their bioactivity.
7 Department of Polymer Chemistry, Graduate School of Engineering, Kyoto University, Kyotodaigaku Katsura, Kyoto 615-8510, Japan.6 Department of Oral and Maxillofacial Surgery, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo 113-8549, Japan.5 Department of Oral Prosthetic Engineering, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo 113-8549, Japan.4 Department of Oral Biology, Faculty of Dentistry, Damanhour University, Damanhour 22511, Egypt.3 Department of Dentistry, Oral and Maxillofacial Surgery, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke 329-0498, Japan.2 Department of Oral Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo 113-8549, Japan.1 Department of Basic Oral Health Engineering, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo 113-8549, Japan.
0 kommentar(er)
